Barnsley fear that centre back Peter Ramage could be sidelined for six weeks with a thigh muscle tear.
The 29 year old who is on a season long loan from Crystal Palace had to be helped off in the 67th minute of the 2-0 defeat at Queens Park Rangers on Saturday and could now be set for a lengthy spell on the sidelines.
Reds boss David Flitcroft is set to turn to either Bobby Hassell or the fit again Lewin Nyatanga.