The Reds took the lead through a Chris Shuker goal on the half hour but couldn't hold onto the three points despite fielding a strong line-up.
John Eustace scored Stoke's equaliser after 81 minutes.
BARNSLEY LINE-UP: Colgan, Austin, Farmery, Laight, Atkinson, Burns (Heslop 45), Baker, Shuker (Cooke 45), Boulding, Joynes (Harban 78), Onibuje.
