Prior to the game Burton had faced Barnsley five times in the Football League without winning, more than any other opponent they've faced without picking up a victory.
It was the seventh time Barnsley and Burton have met in all competitions, and the first time the Brewers have ever led.
It is also the first time ever Burton have scored more than once at Oakwell.
The Reds were overtaken by Burton in the Championship table with this result, dropping to second bottom. They now have just one win in 18 in all competitions, dating back to the start of November (W1 D6 L11) and have lost four of their last five league matches, failing to win any of the last six.
The first goal of the Jose Morais era was the earliest Barnsley have conceded since October 2014 when Jason Kennedy netted after just 44 seconds for Bradford.
There have now been 16 half time substitutions made by Barnsley this season, compared to just five in the entire 2016-2017 campaign.
Jose Morais is the first permanent Barnsley manager (or who would go onto be permanent following a caretaker spell) to lose his first match in charge since David Flitcroft who lost 2-1 at Peterborough.
Dani Pinillos scored his first goal since March 2017 and only his second ever in English football.
There were 11,774 in attendance, the biggest Oakwell midweek night crowd since August (2-1 win over Nottingham Forest)
Nigel Clough won a Championship match at Oakwell for the first time (P6 W1 D4 L1)
The opposition has opened the scoring at Oakwell on nine occasions this season - the most in a campaign since 2015-2016 (13). Barnsley themselves have not scored the first goal at home for ten matches, since their last home win, against Birmingham in November.
Barnsley remain one of four Championship sides this season to have failed to win after going behind, along with Bolton, Reading and Sunderland.
The club record without scoring more than once in a game continues and now stands at 18 successive matches.
They have won only one of their first eight games of 2018 - their worst start to a calendar year since 2014 (also 1 win from the first 8) and the Reds were relegated at the end of that 2013-2014 season.
Barnsley have now played 40 home league games since winning promotion back to the Championship, and won only nine. (W9 D17 L14) - they have failed to win any of the last nine, equalling their worst home run of last season.
They have won only three times at Oakwell this season in the Championship, only Sunderland and Burton (both 2) have won less in front of their own supporters.
Nick Townsend made his 13th start for Barnsley, he has kept just one clean sheet and been on the winning side only three times (This is the eighth successive game between the sticks for Townsend in which Barnsley have not won)
Despite defeat Barnsley enjoyed 60% possession, their second highest of the season - enjoyed 61% also in defeat, against Aston Villa in September.
This was Burton's first win in eight matches, both of their last two wins (achieved across 11 games) have both arrived in South Yorkshire (Hillsborough and Oakwell) Each of the Brewers last five victories have all been achieved away from home.
They had only five shots on goal at Oakwell, the lowest by an opponent there this season.
Burton also failed to win a single corner, the first Barnsley opponent to fail to win a corner this season.
Mamadou Thiam (for Isgrove 45)
Brad Potts (for Hammill 57)
Kieffer Moore (for Bradshaw 72)
Adam Davies, George Moncur, Dimitri Cavare, Adam Jackson.
Stephen Bywater, Lucas Akins, Tom Naylor, Kyle McFadzean, Lloyd Dyer, Jacob Davenport, Martin Samuelson, Jamie Allen, Hope Akpan, Joe Sbarra, Liam Boyce.
Darren Bent (for Boyce 71)
Jake Buxton (for Sbarra 84)
Luke Varney (for Allen 88)
Luke Murphy, Damien McCrory, Harry Campbell, Shaun Barker.
Burton Albion coach Andy Garner:
"We deserved it tonight, especially with the first-half performance. We are absolutely delighted with the result.
"It was a game of two halves. We knew there would be a response in the second half and 45 minutes was a long time hanging in there.
"It was total bombardment, but thankfully we got the three points.
"Perhaps they were a little shocked at how well we played in the first 15 minutes. That's as good as we've played for a long, long time."
Sky Bet Championship results:
Tuesday 20th February 2018:
Aston Villa 1-1 Preston North End
Grabban pen 66 Barkhuizen 37
BARNSLEY 1-2 Burton Albion
Pinillos 75 Allen 1, Davenport 45+4
Brentford 5-0 Birmingham City
Roberts og 54
Middlesbrough 3-1 Hull City
Gestede 16,58 Evandro 41
Millwall 2-1 Sheffield Wednesday
Gregory 52 Pelupessy 42
Nottingham Forest 1-1 Reading
Tomlin 85 Richards 35
Sheffield United 2-1 Queens Park Rangers
Stearman 27 Freeman 63
Bolton Wanderers 1-0 Sunderland
Wednesday 20th February 2018:
Bristol City 1-1 Fulham
Reid 35 Mitrovic 14
Derby County 2-2 Leeds United
Weimann 45+2 Lasogga 34, Alioski 79
Ipswich Town 0-1 Cardiff City
Wolverhampton Wanderers 2-2 Norwich City
Lewis og 14 Zimmermann 27, Oliveira 90+3
Barnsley lie in 23rd place in the Sky Bet Championship with 27 points from 32 games.
We are 26 points outside the Play-Offs, 36 points off the Automatic Promotion Places, 45 points off First place and 1 point above the Relegation Zone.
Up to 21/02/18
On The Road:
Sky Bet Championship Matchday Thirty Three: Birmingham City v Barnsley: Saturday 24th February 2018. Kick-Off: 3pm
Sky Bet Championship Matchday Thirty Three: Barnsley v Norwich City, Saturday 3rd March 2018. Kick-Off: 3pm