Barnsley’s long unbeaten run ended one game short of equaling the club record as they lost at Burton Albion for the first time ever.
Three of Barnsley's eight defeats this season in all competitions have now been by a 3-1 scoreline - at Blackpool in the EFL Cup and also Shrewsbury in the league back in October.
The Reds went behind after just five minutes, the earliest they have conceded since that 3-1 reverse at Shrewsbury where they fell behind after two minutes.
It was the first time in 12 matches that Barnsley have conceded the opening goal in a game, since the 2-2 draw at Oxford at the end of January.
Barnsley have conceded five goals in their last two matches - prior to this they conceded five in 12.
It is the first time the Reds have conceded more than once in successive games since October when they lost 2-0 at Charlton and then 3-1 at Shrewsbury.
It is the eighth time in 41 league games that Barnsley have conceded more than once and is the third time this season they have conceded more than twice - also at Shrewsbury and Sunderland.
Despite this Barnsley do still have the best defensive record in the Football League (33 conceded), one goal less than League One leaders Luton.
Burton sealed the game with goals after 81 and 89 minutes, the first time Barnsley have conceded twice in the last ten minutes of a match since April 2018 when Bolton netted after 82 and 85 in a 2-2 draw at Oakwell.
It is also the first time since that match that there has been three goals in the last ten minutes of a Barnsley game after Cauley Woodrow netted an 85th penalty yesterday (Oli McBurnie netted a last minute equaliser v Bolton)
This was the 13th game Woodrow has scored in for Barnsley this season (all comps) and the first time the Reds have lost.
This was the first time Barnsley have ever lost at Burton (from four visits) and the first time they have even been behind in a game there.
Barnsley were behind at half-time for the seventh time in the league this season and they have never come back to win.
Indeed they have failed to win any of the last THIRTY SEVEN league games in which they have been trailing at half time. last doing so at Crewe in February 2016 when they turned a 1-0 half time deficit into a 2-1 win.
Burton Albion have recorded three consecutive league victories for the first time since April 2018, when they won three in a row and then lost at Preston on the final day to be relegated.
They are the first team to score against Barnsley on their home patch in five games - thus denying the Reds from equaling a club record of five away league games without conceding.
This was also Barnsley's first league defeat of 2019 having previously gone unbeaten in their first 16 league games of a calendar year for the first time since 1933-1934.
1. Adam Davies (C)
23. Dani Pinillos
6. Liam Lindsay
5. Ethan Pinnock
12. Dimitri Cavare
8. Cameron McGeehan
27. Alex Mowatt
33. Jacob Brown
26. Mamadou Thiam
7. Ryan Hedges
9. Cauley Woodrow
21. Mike Bähre (for Thiam 59)
20. Callum Styles (for Brown 59)
29. Victor Adeboyejo (for Pinillos 84)
13. Jack Walton, 18. Adam Jackson, 22. Jordan Willams, 28. Ben Williams.
40. Bradley Collins
2. John Brayford
5. Kyle McFadzean
3. Jake Buxton (C)
26. Colin Daniel
4. Jamie Allen
7. Scott Fraser
23. Stephen Quinn
16. Marcus Harness
27. Liam Boyce
10. Lucas Akins
12. Ben Fox (for Fraser 78)
22. Kieran Wallace (for Allen 90+1)
18. Will Miller (for Boyce 90+4)
1. Stephen Bywater, 15. Alex Bradley, 19. Reece Hutchinson, 21. Josh Clarke.
Burton manager Nigel Clough said:
"It was a very high standard League One game and you can see why Barnsley are right up there challenging for automatic promotion as they are such a good team. They press you home and away the same and put you under a lot of pressure. But we withstood it and caused them problems when we had the opportunity.
"We started brilliantly, got the goal and could have had a penalty when Liam Boyce was booked. We thought it was handball just before that and then Liam went for the second ball and I think nine times out of ten you get those.
"We got the second goal, looked like we were cruising and then there is a penalty against you and we are still not sure what it is for.
"Despite one or two decisions going against us today we still got the three points and just about deserved it."
League One results:
Saturday 6th April 2019:
AFC Wimbledon 1-1 Accrington Stanley
Bradford City 0-1 Doncaster Rovers
Burton Albion 3-1 BARNSLEY
Woodrow pen 85
Fleetwood Town 2-2 Southend United
Evans pen 84
Luton Town 2-2 Blackpool
Peterborough United 2-0 Gillingham
Maddison pen 57
Plymouth Argyle 0-2 Charlton Athletic
Canavan og 49
Rochdale 1-2 Sunderland
Shrewsbury Town 1-1 Scunthorpe United
Hammill og 84
Walsall 1-3 Oxford United
Wycombe Wanderers 2-3 Portsmouth
Sunday 7th April 2019:
Coventry City 0-0 Bristol Rovers
Barnsley remain in 2nd place in Sky Bet League One with 79 points from 41 games. We are 18 points inside the Play-Offs, Goal Difference inside the Automatic Promotion Places, 8 points off First Place and 36 points above the Relegation Zone.
Up to 07/04/19
Sky Bet League One Matchday Forty Two: Barnsley v Fleetwood Town, Saturday 13th April 2019. Kick-Off: 3pm
On The Road:
Sky Bet League One Matchday Forty Four: Plymouth Argyle v Barnsley, Monday 22nd April 2019. Kick-Off: 3pm